Assembly mechanism of the α-pore–forming toxin cytolysin A from Escherichia coli

Soluble Oligomers of the Pore-forming Toxin Cytolysin A from Escherichia coli Are Off-pathway Products of Pore Assembly.

The α-pore-forming toxin Cytolysin A (ClyA) is responsible for the hemolytic activity of various Escherichia coli and Salmonella enterica strains. Soluble ClyA monomers spontaneously assemble into annular dodecameric pore complexes upon contact with membranes or detergent. At ClyA monomer concentrations above ∼100 nm, the rate-limiting step in detergent- or membrane- induced pore assembly is th...

The mechanism of action of Escherichia coli heat-stable toxin.

Holmgren, J. (1981) Nature (London) 292, 413417 Hughes, J . M., Murad, F., Chang, B. & Guerrant, R. L. (1978) Nature (London) 271, 755-756 Hughes, S., Warhurst, G., Turnberg, L. A,, Higgs, N. B., Giugliano, L. G. & Drasar, B. S. (1983) Gut 24 (2), 94-98 James, C., Dibley, M., Burke, V., Robinson, J. & Gracey, M. (1982) Clin. Expt. Immunol. 47, 3 4 4 2 Leppla, S. H. (1982) Proc. Natl Acad. Sci. ...

Cloning of Clostridium perfringens iota toxin gene in Escherichia coli

Iota toxin is produced by Clostridium perfringens type E. This toxin causes antibiotic-associated enterotoxemia in lambs and calves. Iota toxin is a binary toxin that has two components including Ia (the enzyme component) and Ib (the binding component). Ib binds to the surface receptor of target cells and translocate Ia into the cytosol of cells. The aim of this study was to clone toxigenic epi...

Cloning of Binding Domain of Clostridium botlllinum Toxin Type A in Escherichia coli

The heat-stable toxin I gene from Escherichia coli 18D.

The heat-stable toxin I gene in the human Escherichia coli isolate 18D is the estA1 allele. The gene is not part of a composite transposon, but inspection of the flanking DNA sequences suggests that it was at one time part of a transposon. The hypothetical transposon originated from an event other than the occurrence that formed Tn1681.